Sweet Potato Protein exerts significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo

Sweet potato protein is not only beneficial for hormonal balance, but also for cancer control. In this perspective, this protein was shown to slow down the growth of colon cancer cells by 65% in vitro, and by 58 percent in vivo, when given to mice. This protein also inhibited lung tumors by 50% in the same mice. Other studies have also shown cancer inhibition, from liquid cancers like lymphomas to solid cancers like breast and kidney malignancies.  Furthermore, it is well known that sweet potatoes are rich in beta-carotene, vitamin C, manganese and other minerals while they can improve insulin sensitivity, so that glucose can cross cellular membranes with greater ease. Raw is best. But steamed can be acceptable. Either as they are or with a beychamel sauce made from omega 3 rich oils (eg flax and walnut), olive oil, red palm  oil, nutritional yeast (rich in B-12), coconut milk, sea salt, sea vegetables and medicinal aromates.
World J Gastroenterol. 2013 Jun 7;19(21):3300-8.
Anticancer effects of sweet potato protein on human colorectal cancer cells.
Li PG1, Mu TH, Deng L.
Abstract
AIM:
To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines.
METHODS:
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33258 nuclear staining and Boyden transwell chamber methods were used to determine whether purified sweet potato protein (SPP) from fresh sweet potato roots affected proliferation, migration and invasion, respectively, of human colorectal cancer SW480 cells in vitro. The inhibitory effects of SPP on growth of human colorectal cancer HCT-8 cells intraperitoneally xenografted in nude mice and spontaneous lung metastasis of murine Lewis lung carcinoma 3LL cells subcutaneously transplanted in C57 BL/6 mice were also investigated in vivo.
RESULTS:
SPP inhibited the proliferation of SW480 cells in a dose-dependent manner, with an IC50 value of 38.732 μmol/L (r (2) = 0.980, P = 0.003) in the MTT assay. Hoechst 33258 nuclear staining further revealed inhibition of cell viability and induction of apoptosis by SPP. The transwell assay disclosed significant reduction in migrated cells/field by 8 μmol/L SPP (8.4 ± 2.6 vs 23.3 ± 5.4, P = 0.031) and invaded cells/field through the ECMatrix by 0.8 μmol/L SPP, compared with the control (25.2 ± 5.2 vs 34.8 ± 6.1, P = 0.038). Both intraperitoneal (ip) and intragastric (ig) administration of SPP led to significant suppression of growth of intraperitoneally inoculated HCT-8 cells in nude mice to 58.0% ± 5.9% (P = 0.037) and 43.5% ± 7.1% (P = 0.004) of the controls, respectively, after 9 d treatment. Bloody ascites additionally disappeared after ip injection of trypsin inhibitor. Notably, ig and ip administration of SPP induced a significant decrease in spontaneous pulmonary metastatic nodule formation in C57 BL/6 mice (21.0 ± 12.3 and 27.3 ± 12.7 nodules/lung vs 42.5 ± 4.5 nodules/lung in controls, respectively, P < 0.05) after 25 d treatment. Moreover, the average weight of primary tumor nodules in the hind leg of mice decreased from 8.2 ± 1.3 g/mice in the control to 6.1 ± 1.4 g/mice in the ip group (P = 0.035).
CONCLUSION:
Sweet Potatoe protein exerts  significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo.

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Holistic Oncology Movie’s Founder, Christian Joubert has worked as a professor of public law and holistic medicine for most of his adult life, in association with his activities in organic agriculture. Christian’s has attained expertise in many fields, thanks to which he can help as many people as possible, including, but not limited to biogerontology, (ie, extending healthy human life-spans to over 120 years old), holistic oncology, traditional naturopathy (or what is also called “natural medicine”, coming from Hippocratic Tradition), French medicine, (classified by the W.H.O. as the number one out of in 191 countries, Cf. the link “about”), comparative public policy and international public law. Trained in conventional medicine, acupuncture, naturopathy, oenology, the social sciences and law in France, Christian has campaigned and litigated in Courts of law for multiple years in order to replace outdated medical standards of care with evidence-based holistic standards. His originality is based on being able to examine medicine and the health sciences with legal & forensic eyes, thanks to which he can distinguish the relevant data from the irrelevant. In addition, his broad knowledge relative to the health sciences, organic farming, ecology, eco-development, oenology (wine science), sociology, political science, the law and different cultures allows him to identify those key inter-connections that too often, most family members, medical doctors, professors of medicine and public health experts are not able to see or understand. To benefit in vivo from Christian’s trans-cultural, medical, legal and inter-disciplinary training and experience, click the Consult link in the top menu bar.

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