Sweet Potato Protein exerts significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo

Sweet potato protein is not only beneficial for hormonal balance, but also for cancer control. In this perspective, this protein was shown to slow down the growth of colon cancer cells by 65% in vitro, and by 58 percent in vivo, when given to mice. This protein also inhibited lung tumors by 50% in the same mice. Other studies have also shown cancer inhibition, from liquid cancers like lymphomas to solid cancers like breast and kidney malignancies.  Furthermore, it is well known that sweet potatoes are rich in beta-carotene, vitamin C, manganese and other minerals while they can improve insulin sensitivity, so that glucose can cross cellular membranes with greater ease. Raw is best. But steamed can be acceptable. Either as they are or with a beychamel sauce made from omega 3 rich oils (eg flax and walnut), olive oil, red palm  oil, nutritional yeast (rich in B-12), coconut milk, sea salt, sea vegetables and medicinal aromates.

World J Gastroenterol. 2013 Jun 7;19(21):3300-8.

Anticancer effects of sweet potato protein on human colorectal cancer cells.
Li PG1, Mu TH, Deng L.

Abstract

AIM:

To investigate the effects of proteins purified from sweet potato storage roots on human colorectal cancer cell lines.

METHODS:

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst 33258 nuclear staining and Boyden transwell chamber methods were used to determine whether purified sweet potato protein (SPP) from fresh sweet potato roots affected proliferation, migration and invasion, respectively, of human colorectal cancer SW480 cells in vitro. The inhibitory effects of SPP on growth of human colorectal cancer HCT-8 cells intraperitoneally xenografted in nude mice and spontaneous lung metastasis of murine Lewis lung carcinoma 3LL cells subcutaneously transplanted in C57 BL/6 mice were also investigated in vivo.

RESULTS:

SPP inhibited the proliferation of SW480 cells in a dose-dependent manner, with an IC50 value of 38.732 μmol/L (r (2) = 0.980, P = 0.003) in the MTT assay. Hoechst 33258 nuclear staining further revealed inhibition of cell viability and induction of apoptosis by SPP. The transwell assay disclosed significant reduction in migrated cells/field by 8 μmol/L SPP (8.4 ± 2.6 vs 23.3 ± 5.4, P = 0.031) and invaded cells/field through the ECMatrix by 0.8 μmol/L SPP, compared with the control (25.2 ± 5.2 vs 34.8 ± 6.1, P = 0.038). Both intraperitoneal (ip) and intragastric (ig) administration of SPP led to significant suppression of growth of intraperitoneally inoculated HCT-8 cells in nude mice to 58.0% ± 5.9% (P = 0.037) and 43.5% ± 7.1% (P = 0.004) of the controls, respectively, after 9 d treatment. Bloody ascites additionally disappeared after ip injection of trypsin inhibitor. Notably, ig and ip administration of SPP induced a significant decrease in spontaneous pulmonary metastatic nodule formation in C57 BL/6 mice (21.0 ± 12.3 and 27.3 ± 12.7 nodules/lung vs 42.5 ± 4.5 nodules/lung in controls, respectively, P < 0.05) after 25 d treatment. Moreover, the average weight of primary tumor nodules in the hind leg of mice decreased from 8.2 ± 1.3 g/mice in the control to 6.1 ± 1.4 g/mice in the ip group (P = 0.035).

CONCLUSION:

Sweet Potatoe protein exerts  significant antiproliferative and antimetastatic effects on human colorectal cancer cell lines, both in vitro and in vivo.

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